Reporter

Part:BBa_K199077:Design

Designed by: Clifton Davis   Group: iGEM09_MoWestern_Davidson   (2009-09-20)


RBS RFP with CUAGC Addition


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 21
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 578
    Illegal AgeI site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

The template of the gene comes from Part BBa_E1010. We designed a forward primer that included RBS,ATG, the 5-bp addition CTAGC and the first 20 nucleotides on the 5' end of RFP after ATG. This made the edited 5' sequence for our RFP.

References